AKG SHGL-18MN-R Manuel d'utilisateur

Users Manual Fluoview Scanning Laser Biological Microscope Ver. 1.1 WARNING A Caution: Before using your microscope, the items in this manual Identifi

2. Warning Labels IX Configuration The laser beam is emitted (3) from the objective. (3) The laser beam is emitted from the condenser lens. 2-4

2. Warning Labels 2-1-3 Protective Housing Label (4) CAUTION - Laser radiation when open. DO NOT STARE INTO BEAM t/-^f3tS®^ajit«L»c t (5) DANGER - Las

2. Warning Labels IX configuration Ar : (4) Kr + Ar : (5) Do not remove the cube tuaet. When the transmitted detection unit is not mounted (left) and

2. Warning Labels Common to all configurations When the slider cover is removed (6) Do not remove the detection mode selector slider. In addition to t

3. Precautions for Use of the System 3 Precautions for Use of the System Installation • The FLUOVIEW system will be assembled and setup by qualified t

3. Precautions for Use of the System • Standard objectives for biological use are employed for both the upright microscope system configuration (BX) a

Concerning This Section This section provides an overview of the Fluoview System and should be read before the system is used. After reading and under

Contents CAUTIONS 2 REGISTERED TRADEMARKS 2 CARE AND MAINTENANCE 3 MOVING THE FLUOVIEW 3 SECTION 1 INTRODUCTION H 1-1 Fluoview Manual Configuration 1-

Cautions (1) This software and manual may not be reproduced in part or in their entirety without the express written permission of Olympus. (2) The co

Care and Maintenance (1) When not in use, always use the accessory cover to protect your microscope from dust. (2) This microscope is a precision inst

raj ?3 n IF=° -About This Manual This manual explains potential risks and provide important safety information designed to ensure the safe use bf the

Section 1 Introduction Section 1 Introduction 1-1 Fluoview Manual Configuration There are two Fluoview manuals; the Users Manual and the On-screen Man

Section 1 Introduction 1-2 Conventions Used in This Manual The following is an explanation of the various conventions used in this manual. <• <•

^ Section 1 Introduction > Terms Unique to This System Convention Explanation XY obsen/ation This means using XY scan to obsen/e. (Other observatio

Section 2 System Overview Section 2 System Overview The Olympus Fluoview is a confocal scanning type laser fluorescent microscope that utilizes a comm

Section 2 System Overview 2-2 Fluoview Features 1. The detector has a resolution of 12 bits, so images are extremely clear, 2. 1 A high-resolution 102

Section 2 System Overview 2-3 Optical Path Diagram CHI CH2 FL TR Laser CH2 CH2 CHI CH2 TR FL CH2 PM2 • — 2-3

Section 2 System Overview 2-4 System Configuration 2-4-1 System Units and Their Roles Scan unit This unit scans the laser beam in the X and Y directio

Section 2 System Overview Microscope A BXWI fixed stage erect image microscope for fluorescent light observation. VIbration-free stand A special pneum

Section 2 System Overview Microscope AN 1X70 Inverted Image microscope for fluorescent light observation. Vibration-free stand A special pneumatic typ

Section 2 System Overview 2-5 Software Function Configuration This software uses panel type windows. With conventional software, it is necessary to se

1 Safety Precautions 2 Warning Labels A CONTENTS 1-1 2-1 2-1 Warning Labels Concerning Laser Safety 2-1 2-1-1 Warning Labels 2-1 2-1-2 Aperture Label

Section 2 System Overview 2-5-2 Function Panels and Display Panels The Fluoview software utilizes two types of panels: the function panel and the disp

Section 2 System Overview 2-5-3 Drag and Drop Function Execution Icon This software uses the drag and drop method of selecting image files and observa

Section 2 System Overview 2-6 System Setup This system must be set up by a special technician. To maintain the perfonnance and for safety, never disas

Section 2 System Overview 2-6-2 Turning on the Power Supply Tum on the power supply of each unit. When only reading data, the laser power supply and i

Section 2 System Overview • Tuming on the power controller power supply (1) Tum on the main switch. For details, refer to the Power Controller SSI-950

Section 2 System Overview Tuming on the control unit power supply (1) Turn on the power supply switch. This unit is connected to the power controller,

Section 2 System Overview 2-7 System Operational Procedure Overview A flow chart is used here to explain the operational procedures so that operation

Section 2 System Overview 2-7-1 Fluorescent Light Observation Procedure Start the system. Prepare the system (tum on the power supply). Introduction t

Section 2 System Overview 2-7-2 Transmitted Light Observation Procedure start the system. Prepare the system (tum on the power supply). Introduction t

Section 2 System Overview 2-8 Identifying Images From Different Methods of Observation Fluoview displays various image icons which can be used to iden

1. Safety Precautions 1 Safety Precautions A Depending on the laser configuration, this laser product is classified as follows: Ar laser configuration

This instmction manual describes functions, specifications, assembly (connections) and adjustment of the hardware. Before reading this instmction manu

LASER SAFETY PRECAUTION Units indicated as "OPERATOR SERVICE" should be removed only by a person who has had laser safety training, and only

1 STANDARD CONFIGURATIONS 1 STANDARD CONFIGURATIONS 1-1 BX Upright Microscope System Configuration bfesi&;SftM!3escnpbonMH?#g^ Scan Unit Excitatio

CONTENTS 1 STANDARD CONFIGURATIONS t£t 1-1 BX Upright Microscope System Configuration 1-1 1-2 IX Inverted Microscope System Configuration 1-2 1-3 Opti

1 STANDARD CONFIGURATIONS 1-2 IX Inverted Microscope System Configuration ^nimsii^ Scan Unit Excitation Cube Filter Dual Wavelength Excitation Cube Ba

1 STANDARD CONFIGURATIONS 1-3 Optional Accessories Green HeNe Laser Unit Dual Wavelength Excitation ~ Cube Banier Filter Transmitted Light Detector fo

2 MAIN UNITS AND DESCRIPTION OF CONTROLS 2 MAIN UNITS AND DESCRIPTION OF CONTROLS Also referto Section 2-4, SYSTEM LAYOUT in the "INTRODUCTION TO

2 MAIN UNITS AND DESCRIPTION OF CONTROLS In general, selecting a smaller pinhole than the pinhole size recommended for a given objective will not incr

2 MAIN UNITS AND DESCRIPTION OF CONTROLS (2) Detection mode selector slider Slider for selecting either two fluorescence signals or one fluorescence s

2 MAIN UNITS AND DESCRIPTION OF CONTROLS Laser Slider at pushed-in position CHI CH2 FL TR Fig. (I) Middle position Fig.(n) Slider at pulled-out positi

1. Safety Precautions If the objective revolving nosepiece or the objective lens is removed during laser output (during laser scanning), the laser bea

2 MAIN UNITS AND DESCRIPTION OF CONTROLS 1 (3) Bamer filter slider Up to two filters can be placed for both CHI and CH2. At the pushed-in position, th

2 MAIN UNITS AND DESCRIPTION OF CONTROLS • FITC emission tails longer than 570 nm. Accordingly, FITC fluorescence may be detected on the CH2 where it

2 MAIN UNITS AND DESCRIPTION OF CONTROLS 2-2 Microscope Frame The illustration below shows the main controls of the microscope frame. The stage, revol

2 MAIN UNITS AND DESCRIPTION OF CONTROLS (1) Light path selector • When the knob is pushed-in, visual observation is possible. • When the knob is pull

2 MAIN UNITS AND DESCRIPTION OF CONTROLS (2) Universal condenser For Nomarski observation, engage the Nomarski prism (optional) suitable for the objec

2 MAIN UNITS AND DESCRIPTION OF CONTROLS IX Confiquration (6) Filter "OPERATOR SERVICE" objective lens (1) Light path selector "OPERATO

2 MAIN UNITS AND DESCRIPTION OF CONTROLS (1) Light path selector • When tiie knob is set at the ^[ position, visual observation is possible. • Set the

2 MAIN UNITS AND DESCRIPTION OF CONTROLS (2) Condenser For Nomarski obsen/ation, engage the Nomarski prism (optional) conesponding to the objective in

2 MAIN UNITS AND DESCRIPTION OF CONTROLS 2-3 Transmitted Light Detector (Optional) FVX-TD-BX (for BX50, BXWI) (1) Light paUi selector • When the knob

3 PREPARATIONS FOR OBSERVATION 3 PREPARATIONS FOR OBSERVATION This section explains the order of procedures for specimen obsen/ation. 3-1 Fluorescence

1. Safety Precautions A Please note that the laser beam is emitted when the display's function pane! ([Acquire] panel) looks as shown below. &quo

3 PREPARATIONS FOR OBSERVATION 3-1-1-2 IX Configuration 1. Turn the light paUi selector dial (1) on ttie right side of Uie microscope to the ^ positio

3 PREPARATIONS FOR OBSERVATION 3-1-2 Selecting the LSM Light Path 3-1-2-1 BX50, BXWI Configuration 1. Pull out the light path selector (1) on the trin

3 PREPARATIONS FOR OBSERVATION 3-1-2-2 IX Configuration 1. 2. 3. 4. Tum the light path selector (1) to the [[ SP J ] position. Set the magnification s

3 PREPARATIONS FOR OBSERVATION 3-1-3 Selecting the Pinhole Operate the pinhole tun-et (1) to select ttie pinhole corresponding to tiie pinhole number

3 PREPARATIONS FOR OBSERVATION (5) FIKer slider cover screws (2) [}etection mode selector slider UBBt IMTEI6ITY 0 (4) ND filter turret (7) Laser line

3 PREPARATIONS FOR OBSERVATION 3-2 Transmitted Observation The procedures for transmitted observation are similar to those for fluorescence observatio

3 PREPARATIONS FOR OBSERVATION 3-2-3 Selecting the LSM Light Path 3-2-3-1 BX50, BXWI Configuration 1. Pull out Uie light path selector (1) on Uie trin

3 PREPARATIONS FOR OBSERVATION 3-2-3-2 IX Configuration 1. Tum the light path selector (1) to select the [[ sP W setting. 2. Set the magnification sel

3 PREPARATIONS FOR OBSERVATION 3-2-4 Selecting the Detection Mode When the detection mode selector slider (2) is: • Pushed in, transmitted light is de

3 PREPARATIONS FOR OBSERVATION 3-2-5 Disengaging the Barrier Filter Remove all the barrier filters from the channel to detect transmitted light. (The

2. Warning Labels 2 Warning Labels 2-1 Warning Labels Concerning Laser Safety 2-1-1 Warning Labels (1) Ar laser configuration ©AUTIOff^ LASER RADIATIO

4 SPECTRAL CHARACTERISTICS OF FILTERS 4 SPECTRAL CHARACTERISTICS OF FILTERS Barrier filters BA510IF BA585IF Representative filter combinations for Ar

4 SPECTRAL CHARACTERISTICS OF FILTERS • Representative filter combination for the Kr and Ar laser combination DM488/568 \ , BA510IF 100 50 -450 Excita

5 SPECIFICATIONS 5 SPECIFICATIONS -:^^M^:r^M^^M^'^:^ir^'$^ Laser unit Scan unit Control unit Ar laser Kr laser Scan mode Laser input Shutter

5 SPECIFICATIONS 5 SPECIFICATIONS ;:Ǥp^p5if^6^^ipjKfi|ps^?i^^ Laser unit Scan unit Control unit Ar laser Kr laser Scan mode Laser input Shutter ND fi

5 SPECIFICATIONS Microscope fi-ame [Upright] BX50-FLA-FVX BXWI-FLA-FVX [Inclined] IX70-FLA-FVX Stand for BX Computer system Power controller Z-motor C

5 SPECIFICATIONS Operating environment Indoor use. Altitude: Max. 2000m Ambient temperature: 5°C to 40t (41* F to 109* F) 10°C to35t (Performance guar

II mil inie^QQinij Q ^' Concerning This section explains countermeasures to be taken in the event that a problem occurs. Please read this section

Contents Section 1 Problem Countermeasures 1-1

Section 1 Problem Countermeasures Section 1 Problem Countermeasures Due to Uie usage, the functions of the system sometimes are not fully effective, a

Section 1 Problem Countermeasures ^^K^^^^S 1. No fluorescent light image. 2. No transmitted light image. 3. Images are disrupted. 4. Visibility is poo

2. Warning Labels IX configuration (Dor (2) 2-2

Section 1 Problem Countermeasures ^^^«^ 6. Flare is visible. 7. Circular flares appear in the center of the image. 8. Blun-ing is visible. 9. Images a

Section 1 Problem Countermeasures teg5l3i?^tSymptomMs4;!Si;#Si 13. The scale of tiie [Z Stage] in the [Acquire] panel is not woridng. 14. The fine mov

2. Warning Labels 2-1-2 Aperture Label (Indicates where the laser beam is emitted.) (3) AVOID EXPOSURE Laser radiation is emitted from this aperture B